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Lectures and Labs: Principles of Genetics BIOL 362

The below schedule is subject to daily change. Assignments and handouts will be added as the class progresses.
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Date

Lecture Topic

Reading

Week

Lab activities

Fri. Sept 4 Introduction Handout, Slides
Ch 1
Mon. Sept 7 Labor Day, no class
Sept 7-9 No lab this week
Wed. Sept 9 Chromosomes and cellular reproduction Handout, Slides
Cell Cycle, Meiosis, Genetic variation and meiosis,Genetic variation and meiosis
Ch 2, p.13-34
Fri. Sept 11 Principles of heredity Handout, Slides
Ch 3, p.43-56
Mon. Sept 14 Principles of heredity Handout, Slides
Mendelian Genetics Overview
Science is Real
Ch 3, p. 62-64;
Ch 4, p.81-82,84-87;
Ch 5, p.99-104
Sept 14-16 Lab 1: Meiosis & Mitosis
Computer Flies 1
Fly lab logon
Lab Report 2-4 pages due next week in lab on one set of fly crosses (not one in lab handout)
Wed. Sept 16 2 loci (different chromosomes) Handout, Slides
Ch 3, p.57-65
Fri. Sept 18 Gene interactions, Epistasis Handout, Slides
Ch 5, p.105-115
Mon. Sept 21 Genetic linkage Handout, Slides
Ch 7, p. 160-170 Sept 21-23 Lab 2: Computer Flies 2
Fly lab logon
Identify Drosophila mutants
Wed. Sept 23 Genetic linkage cont, Non-Mendelian Inheritance Handout, Slides
Ch 7, p. 160-175; Ch 5 p. 115-120
Fri. Sept 25 Extensions of basic principles
Ch 5, p.115-120,122-123
Mon. Sept 28 Pedigrees Handout, Slides
Ch 6 Sept 28-30 Lab 3: Computer Flies 3
Fly lab logon
Genetic Disease introduction
Genetic Counselor
Wed. Sept 30 Review/Catchup
Thurs. Oct 1 Review Session
5pm Bunnell 308
Fri. Oct 2 Exam 1
Study Guide
Old Exams
Mon. Oct 5 Mapping Handout, Slides
Mapping with 3 point tetstcross
Ch 7, p.173-185,188-189 Oct 5-7 Lab 4: Fruit DNA extraction
Pipette techniques and safety
Wed. Oct 7 Mapping Handout, Slides
Ch 3
Fri. Oct 9 Mapping Handout, Slides
Ch 24
Mon. Oct 12 Quantitative Genetics, QTLs Handout, Slides
Read about heritability
Ch 24 Oct 12-14 Lab 5: DNA extraction-plants
Genetic Disease Presentations (group project)
Wed. Oct 14 Quantitative Genetics, QTLs Handout, Slides
Ch 24
Fri. Oct 16 Quantitative Genetics,PCR Handout, Slides
Ch 24
Mon. Oct 19 DNA replication, pcr Handout, Slides
DNA replication - simplePCR1, PCR2
Ch19 p.513-515 Oct 19-21 Lab 6: PCR
DNA replication
Wed. Oct 21 QTLs, molecular markers Handout, Slides,
Electrophoresis
Ch 24
Fri. Oct 23 QTLs, hapmap Handout, Slides,
Microarray basics
Microarray Method for Genetic Testing - Detailed
Watch lab processes for high throughput microarray processing

HapMap
Haplotypes
HapMap Wikepedia
Ch 24
Mon. Oct 26 Review/Catchup, midterm evaluations
Oct 26-28 Lab 7: Gel of PCR
PCR cleanup
23 and Me Handout
DNA Diary
23 and me
Tue. Oct 27 Review Session
5pm Bunnell 308
Wed. Oct 28 Exam 2

Study Guide
Old Exams
Fri. Oct 30 DNA sequencing Handout, Slides,

Sanger Sequencing
Mon. Nov 2 Chromosomes Handout, Slides,

Heterozygous Chromosome Inversion effect on meiosis
Heterozygous inversion effect on meiosis
Robertsonian Translocation
Ch 9 Nov 2-4 Lab 8: WRRB tour Meet in Elvey Entrance area DNA sequencing
Wed. Nov 4 Chromosomes Handout, Slides,

Gene duplications and evolution of new functions Click on "8-09 Evolution of homologous genes"
Ch 9
Fri. Nov 6 Chromosomes Handout, Slides,

Mistakes in Meiosis
Ch 9
Mon. Nov 9 transposable elements Handout, Slides,

Cut and paste transposition - click on "simple transposition"
Copy and Paste transposition (replicative transposition)
Ch 11 Nov 9-11 Lab 9: Quiz on DNA sequencing and PCR and reading
Reading to discuss in lab: The second coming of gene therapy
Chromosome rearrangements
Wed. Nov 11 transposable elements Handout, Slides,

Transcription and translation
Transcription and translation 2
Viral reverse transcription
McClintock and Ac Ds transposons"
Ch 11
Fri. Nov 13 Bacterial transformation Handout, Slides,

Restriction enzyme and ligating insert into plasmid
Bacterial transformation Click on "18-02 Steps in cloning a gene"

Plant transformation Click on "19-08 Genes into plants using the Ti plasmid"
Gene Gun
Gene gun 2
Mon. Nov 16 Shotgun sequencing Handout, Slides,

genomic DNA library
Shotgun sequencing
Nov 16-18 Lab 10: Bacterial Transformation
Shotgun sequencing activity
Jaberwocky genome
Quiz on transposable elements and bacterial transformation (read handout)
Wed. Nov 18 DNA packing Handout, Slides,

DNA packing 1
DNA packing 2
DNA packing 3
histone deacetylation
Ch 11
Fri. Nov 20 Genome size, centromeres, telomeres Handout, Slides,

Telomerase 1
Telomerase 2
Telomerase 3
Nobel prize for telomeres and telomerase
Mon. Nov 23 Review/Catchup
Nov 23-25 Lab 11: No lab this week
Tue Nov 24 Review Session
5pm Bunnell 408 (lab room)
Wed. Nov 25 Exam 3

Study Guide
Old Exams
Fri. Nov 27 Thanksgiving Holiday, no class
Ch 19
Mon. Nov 30 DNA sequence analysis
Nov 30-2 Sequence Analysis
[download MEGA software]
[Class Data]
[Class Sequence Alignment in mega file]
[
Arabidopsis Alignment in mega file]
Check transformation results
Wed. Dec 2 Genomics and proteomics
Fri. Dec 4 Genomics and proteomics
Mon. Dec 7 Organelle DNA
Dec 7-9 Lab 12: Gene Therapy
Wed. Dec 9 Epigenetics
Fri. Dec 11 Epigenetics

Epigenetics information Epigenetics video
Epigenetics web site, Epigenetics in Discover Magazine, 1hr Nova Epigenetics Video
Epigenetics Review Paper
Mon. Dec 14 Review/Catchup
Dec 14-16 No lab this week
Wed. Dec 16 10:15-12:15 Final Exam (half on new material, half cumulative)

Study Guide
Old Exams
Instructor contact information:

Diana Wolf
office: West Ridge Research Building (WRRB) 240 (map to office)
email: diana.wolf@uaf.edu
phone: (907) 474-5538
office hours: Mon, Wed 12:45-2 (after class)